Journal: Geromedicine
Article Title: Mutagen-induced somatic mutation rate in primary mammalian cells in relation to maximum life span
doi: 10.70401/geromedicine.2026.0023
Figure Lengend Snippet: (A) Viability of human IMR90 cells after treatment with different doses of ENU. Proliferating cells were treated with ENU doses ranging from 25–500 μg/ml and the number of viable cells was assessed 72 hrs after treatment as a percentage of the control population (1-way ANOVA, R 2 = 0.9747); (B) Percentage of cells in early (Annexin positive) and late stage (7-AAD) apoptosis; (C) Viability of ENU-treated cells when confluent; (D) SNV frequency in human IMR90 cells after treatment with 20 or 100 μg/ml (1-way ANOVA, R 2 = 0.9259). IMR90: Institute for Medical Research-90; ENU: N-ethyl-N-nitrosourea; ANOVA: analysis of variance; 7-AAD: 7-aminoactinomycin D; SNV: single nucleotide variant.
Article Snippet: Commercially available human lung fibroblasts (IMR90) were obtained via ATCC (CCL-186).
Techniques: Control, Variant Assay